What is GFP?

Introduction One of the most important discoveries in the field of fluorescent microscopy was found in a jellyfish in the 1960s. Osamu Shimomura of Princeton University was...

Phase-Contrast Microscopy

Introduction Light microscopy offers a powerful technique for label-free imaging of biological samples such as cells. Label-free imaging is particularly well-placed for understanding more about...

Filters

Introduction The light microscope remains one of the most used tools for research, particularly in the fields of biological or biophysical sciences and offers a...

Rolling vs Global Shutter – White Papers

Introduction CCD/EMCCD and CMOS camera sensor technologies both use electronic shutters, but differ in the way each sensor reads the signal at a given pixel. A...

Anatomy Of A Microscope

Introduction At its core, a typical microscope is essentially a box designed to hold two lenses in precise positions so that light can be accurately magnified from the...

Fluorescence Imaging

Introduction Tissues, cells, and the smaller structures inside cells (organelles) are mostly water and are therefore transparent. Imaging tiny see-through bags of water results in...

Maximizing Microscope Field of View With Scientific Cameras

Introduction Microscope field of view (FOV) is the maximum area visible when looking through the microscope eyepiece (eyepiece FOV) or scientific camera (camera FOV), usually...

What Is Spinning Disk Confocal Microscopy?

Introduction Typical fluorescence microscopy involves illuminating the entire sample and detecting the resulting fluorescence. Illuminating and detecting from the entire sample includes collection of out-of-focus...